Journal: CNS Neuroscience & Therapeutics
Article Title: Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator
doi: 10.1111/cns.14825
Figure Lengend Snippet: RtPA upregulates Piezo1 expression in the cerebral microvasculature of the rats with MCAO. (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells. *** p < 0.001 vs Sham group, ## p < 0.001 vs MCAO group, ### p < 0.001 vs MCAO group. Results are shown as mean ± SD, n = 6 (one‐way ANOVA). (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells.
Article Snippet: Double immunofluorescence labeling for Piezo1 (15939‐1‐AP, Proteintech, USA) and neutrophil cytosolic factor 4 (NCF4) (14648‐1‐AP, Proteintech, USA) was performed, followed by incubation with the secondary antibody and DAPI.
Techniques: Expressing, Labeling, Immunofluorescence, Staining